anti he4 mouse monoclonal antibody  (HyTest)

Journal: Mikrochimica Acta

Article Title: Advantages of stereolithographic 3D printing in the fabrication of the Affiblot device for dot-blot assays

doi: 10.1007/s00604-024-06512-z

Figure Lengend Snippet: a Comparison of the previous CNC-micromachined functionality and the new 3D-printed Affiblot device; an identical calibration procedure was performed on both devices simultaneously; spots of an HE4 antigen were deposited onto the membrane, and two dilutions of two different clones of anti-HE4 antibodies were added to form the immune complexes. b Box charts of the correlations of colorimetric signals to the concentration of the HE4 antigen in the CNC-machined and the 3D-printed device

Article Snippet: Human epididymal secretory protein HE4, anti-HE4 mouse monoclonal antibody (clones 2B13 and 3C24), all HyTest, Ltd., Turku, Finland; HRP-conjugated rabbit anti-mouse anti-IgG antibody, Sigma-Aldrich Corp., St. Louis, MO, USA; rabbit polyclonal anti-endoglin antibody H-300 (lot sc-20632), Santa Cruz Biotechnology, Inc., Dallas, TX, USA; bovine serum albumin (BSA), Sigma-Aldrich Corp., St. Louis, MO, USA; ClarityTM WB ECL Substrate (#1705060), Bio-Rad Laboratories, Hercules, CA, USA; washing buffer (PBS-T, PBS with 0.05% Tween 20), equilibration buffer (10 mM phosphate buffer, pH 7.3), blocking buffer (5% BSA in PBS-T), primary antibody buffer (0.25% BSA in PBS-T), all Penta, Chrudim, Czech Republic.

Techniques: Comparison, Membrane, Clone Assay, Concentration Assay

Journal: Scientific Reports

Article Title: Recurrence monitoring for ovarian cancer using a cell phone-integrated paper device to measure the ovarian cancer biomarker HE4/CRE ratio in urine

doi: 10.1038/s41598-021-01544-4

Figure Lengend Snippet: Comparison between flatbed scanner and cell phone measurement of HE4 concentrations (left) and of creatinine concentrations (right). Error bars the percent error found for each concentration (mean + s.d, n = 4). The dotted line indicates the line of identity. Error bars the percent error.

Article Snippet: Monoclonal mouse anti-human epididymis protein 4 (HE4) was purchased from HyTest (Turku, Finland).

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: Recurrence monitoring for ovarian cancer using a cell phone-integrated paper device to measure the ovarian cancer biomarker HE4/CRE ratio in urine

doi: 10.1038/s41598-021-01544-4

Figure Lengend Snippet: Comparison of mean values for HE4/CRE ratios for scanner and cellphone to actual values (mean + s.d, n = 5 ). The dotted line indicates the line of identity.

Article Snippet: Monoclonal mouse anti-human epididymis protein 4 (HE4) was purchased from HyTest (Turku, Finland).

Techniques:

Journal: Scientific Reports

Article Title: Recurrence monitoring for ovarian cancer using a cell phone-integrated paper device to measure the ovarian cancer biomarker HE4/CRE ratio in urine

doi: 10.1038/s41598-021-01544-4

Figure Lengend Snippet: Simulated clinical patient ratios of urinary HE4/CRE. Each image shows a lateral flow strip and corresponding creatinine strip below. In the LFA strip, the left line is the test line and the right line is the control line. * LMP low malignancy potential cyst. The labels below are the ratio of HE4/CRE and the corresponding stage of ovarian cancer given by Liao et al. (2015) and Hellstrom et al. (2010).

Article Snippet: Monoclonal mouse anti-human epididymis protein 4 (HE4) was purchased from HyTest (Turku, Finland).

Techniques: Stripping Membranes

Journal: Scientific Reports

Article Title: Recurrence monitoring for ovarian cancer using a cell phone-integrated paper device to measure the ovarian cancer biomarker HE4/CRE ratio in urine

doi: 10.1038/s41598-021-01544-4

Figure Lengend Snippet: Schematic of the two paper test strips and components. For the HE4 test strip (top), the sandwich assay captures the biomarker in the middle at the test line. The control line is generated when excess reporter binds. For the creatinine test strip, a two reagent system is used to generate a colorimetric reaction with creatinine from light to dark purple. The intensity of the purple color (bottom) indicates the amount of creatinine . The two strips are placed in a scanner or cassette for the cell phone to analyze.

Article Snippet: Monoclonal mouse anti-human epididymis protein 4 (HE4) was purchased from HyTest (Turku, Finland).

Techniques: Stripping Membranes, Biomarker Assay, Generated

Journal: Scientific Reports

Article Title: Recurrence monitoring for ovarian cancer using a cell phone-integrated paper device to measure the ovarian cancer biomarker HE4/CRE ratio in urine

doi: 10.1038/s41598-021-01544-4

Figure Lengend Snippet: HE4 test line intensity as a function of the assay read time (mean + s.d., n = 2) (left panel). The color intensity as a function of assay read time for three creatinine concentrations of 5 mg/dL, 20 mg/dL, and 40 mg/dL (mean + s.d, n = 3) (right panel).

Article Snippet: Monoclonal mouse anti-human epididymis protein 4 (HE4) was purchased from HyTest (Turku, Finland).

Techniques: Functional Assay

Journal: Scientific Reports

Article Title: Recurrence monitoring for ovarian cancer using a cell phone-integrated paper device to measure the ovarian cancer biomarker HE4/CRE ratio in urine

doi: 10.1038/s41598-021-01544-4

Figure Lengend Snippet: Calibration plots generated from the scanner. ( A ) Standard curve for concentrations of HE4 (pM) (mean + s.d, n = 2). ( B ) Standard curve for concentrations of creatinine (mg/dL) (mean + s.d, n = 3).

Article Snippet: Monoclonal mouse anti-human epididymis protein 4 (HE4) was purchased from HyTest (Turku, Finland).

Techniques: Generated

Journal: Scientific Reports

Article Title: Recurrence monitoring for ovarian cancer using a cell phone-integrated paper device to measure the ovarian cancer biomarker HE4/CRE ratio in urine

doi: 10.1038/s41598-021-01544-4

Figure Lengend Snippet: Overview of the smartphone quantification system. ( A ) Image of the prototype components (lens, cell phone attachments and strip cassette). ( B ) A CAD model providing an overview of the components used in developing the system. ( C ) Schematic of the test-strip holder indicating the color detection scheme and the red boxes (arrows) indicate the region of interest (ROI) for HE4, for background reference, and a ROI for CRE.

Article Snippet: Monoclonal mouse anti-human epididymis protein 4 (HE4) was purchased from HyTest (Turku, Finland).

Techniques: Stripping Membranes

Journal: Scientific Reports

Article Title: Recurrence monitoring for ovarian cancer using a cell phone-integrated paper device to measure the ovarian cancer biomarker HE4/CRE ratio in urine

doi: 10.1038/s41598-021-01544-4

Figure Lengend Snippet: Calibration plots generated from the cell phone-based device. ( A ) Standard curve for concentrations of HE4 (pM) (mean + s.d, n = 2). ( B ) Standard curve for concentrations of creatinine (mg/dL) from cell phone (mean + s.d, n = 3).

Article Snippet: Monoclonal mouse anti-human epididymis protein 4 (HE4) was purchased from HyTest (Turku, Finland).

Techniques: Generated

Journal: Cancer

Article Title: HE4 antigen-autoantibody complexes complement CA125 for detecting early stage ovarian cancer

doi: 10.1002/cncr.32582

Figure Lengend Snippet: (A) HE4 autoantibody; (B) HE4 antigen-autoantibody complexes; (C) HE4 antigen and (D) CA125 antigen. In the upper panels, each dot represents the average of duplicate serum samples from a single case (red) or control (blue). The middle panels contain a box plot where each box represents the maximum, upper quartile, median, lower quartile and minimum values in case and control groups, respectively. The red dashed lines in each plot represent the cut-off value (at 98% specificity) for each biomarker. The lower panel contains a table displaying the sensitivity for cases and controls near 98% specificity.

Article Snippet: Mouse anti-HE4 monoclonal IgG antibody was purchased from OriGene Technologies (Rockville, MD).

Techniques: Control, Biomarker Discovery

Journal: Cancer

Article Title: HE4 antigen-autoantibody complexes complement CA125 for detecting early stage ovarian cancer

doi: 10.1002/cncr.32582

Figure Lengend Snippet: (A) HE4 autoantibody; (B) HE4 antigen-autoantibody complexes; (C) HE4 antigen and (D) CA125 antigen. In the upper panels, each dot represents the average of duplicate serum samples from a single case (red) or control (blue). The middle panels contain a box plot where each box represents the maximum, upper quartile, median, lower quartile and minimum values in case and control groups, respectively. The red dashed lines in each plot represent the cut-off value (at 98% specificity) for each biomarker. The lower panel contains a table displaying the sensitivity for cases and controls near 98% specificity.

Article Snippet: Mouse anti-HE4 monoclonal IgG antibody was purchased from OriGene Technologies (Rockville, MD).

Techniques: Control, Biomarker Discovery

Journal: Cancer

Article Title: HE4 antigen-autoantibody complexes complement CA125 for detecting early stage ovarian cancer

doi: 10.1002/cncr.32582

Figure Lengend Snippet: Complementarity of serum CA125, HE4 antigen and HE4 antigen-autoantibody complexes in early and late stage ovarian cancer

Article Snippet: Mouse anti-HE4 monoclonal IgG antibody was purchased from OriGene Technologies (Rockville, MD).

Techniques:

Journal: Cancer

Article Title: HE4 antigen-autoantibody complexes complement CA125 for detecting early stage ovarian cancer

doi: 10.1002/cncr.32582

Figure Lengend Snippet: (A) Early stage ovarian cancer and (B) Late stage ovarian cancer. A combination of HE4 antigen-autoantibody complexes and CA125 had a significantly greater AUC (0.986) than did CA125 alone (0.879) in early stage disease (P<0.001) and slightly greater AUC (0.985) than did CA125 alone (0.958) in late stage disease (P=0.055). (C) Early stage ovarian cancer from validation sample set. A combination of HE4 antigen-autoantibody complexes and CA125 had a greater AUC (0.965) than did CA125 alone (0.934) in late stage disease P =0.039).

Article Snippet: Mouse anti-HE4 monoclonal IgG antibody was purchased from OriGene Technologies (Rockville, MD).

Techniques: Biomarker Discovery

Journal: Cancer

Article Title: HE4 antigen-autoantibody complexes complement CA125 for detecting early stage ovarian cancer

doi: 10.1002/cncr.32582

Figure Lengend Snippet: (A) HE4 antigen-autoantibody complexes; and (B) CA125 antigen. In the upper panels, each dot represents the average of duplicate serum samples from a single case (red) or control (blue). The middle panels contain a box plot where each box represents the maximum, upper quartile, median, lower quartile and minimum values in case and control groups, respectively. The red dashed lines in each plot represent the cut-off value (at 98% specificity) for each biomarker. The lower panel contains a table displaying the sensitivity for cases and controls near 98% specificity.

Article Snippet: Mouse anti-HE4 monoclonal IgG antibody was purchased from OriGene Technologies (Rockville, MD).

Techniques: Control, Biomarker Discovery

Journal: Cancer

Article Title: HE4 antigen-autoantibody complexes complement CA125 for detecting early stage ovarian cancer

doi: 10.1002/cncr.32582

Figure Lengend Snippet: Fifteen individuals screened on the NROSS trial developed ovarian cancer. Twelve were detected by rising CA125 and the ROCA, whereas three were not. Multiple pre-clinical serum samples from each participant were analyzed for: (A) HE4 autoantibody; (B) HE4 antigen-autoantibody complexes; (C) HE4 antigen; and (D) CA125 antigen. Red dash line represents 98% specificity for each biomarker. Significant increases prior to diagnosis were observed with HE4 autoantibody (P=0.022), HE4 antigen-autoantibody complexes (P=0.010) and CA125 antigen (P=0.031), but not with HE4 antigen (P=0.239).

Article Snippet: Mouse anti-HE4 monoclonal IgG antibody was purchased from OriGene Technologies (Rockville, MD).

Techniques: Biomarker Discovery

Journal: Frontiers in Pharmacology

Article Title: Human Epididymis Secretory Protein 4 (HE4) Compromises Cytotoxic Mononuclear Cells via Inducing Dual Specificity Phosphatase 6

doi: 10.3389/fphar.2019.00216

Figure Lengend Snippet: Genes induced in response to HE4.

Article Snippet: Phenotypes of the clones were evaluated by western blotting using anti-HE4 antibody (Origene, TA801137; ).

Techniques: Variant Assay

Journal: Frontiers in Pharmacology

Article Title: Human Epididymis Secretory Protein 4 (HE4) Compromises Cytotoxic Mononuclear Cells via Inducing Dual Specificity Phosphatase 6

doi: 10.3389/fphar.2019.00216

Figure Lengend Snippet: HE4 upregulates expression of DUSP6 in PBMCs. (A) DUSP6 transcription in response to a 6-hr incubation with 0.01 mg/mL rHE4 (HE4) or equivalent amount of PBS (CTR) were evaluated by triplicated trials of real time PCR using PBMCs from four individual donors. (B) Two-color flow cytometric analysis of PBMC following 24-h incubation with 0.01 mg/mL of rHE4 (HE4) or vehicle (CTR). 2D-scatterplots of DUSP6 (Alexa Fluor 647) and CD3 or CD56 (FITC) are shown. Numbers on the plots indicate mean percentages of the cell populations of the each tetrameric area from four independent experiments. (C) Bar graphs from flow cytometric analyses using PBMCs from four individual donors. The mean ± SEM are shown. * p < 0.05, ** p < 0.01.

Article Snippet: Phenotypes of the clones were evaluated by western blotting using anti-HE4 antibody (Origene, TA801137; ).

Techniques: Expressing, Incubation, Real-time Polymerase Chain Reaction

Journal: Frontiers in Pharmacology

Article Title: Human Epididymis Secretory Protein 4 (HE4) Compromises Cytotoxic Mononuclear Cells via Inducing Dual Specificity Phosphatase 6

doi: 10.3389/fphar.2019.00216

Figure Lengend Snippet: DUSP6 concenrations in cell lysates and culture media of PBMC.

Article Snippet: Phenotypes of the clones were evaluated by western blotting using anti-HE4 antibody (Origene, TA801137; ).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Human Epididymis Secretory Protein 4 (HE4) Compromises Cytotoxic Mononuclear Cells via Inducing Dual Specificity Phosphatase 6

doi: 10.3389/fphar.2019.00216

Figure Lengend Snippet: HE4 upregulates expression of DUSP6 in peripheral CD8 + T cells. Two-color flow cytometric analysis of PBMCs following 24-h incubation with 0.01 mg/mL of rHE4 (HE4) or vehicle (CTR). (A) 2D-scatterplots of DUSP6 (Alexa Fluor 647) and CD4 or CD8 (FITC) are shown. Numbers on the plots indicate mean percentages of the cell populations of the each tetrameric area from four independent experiments. (B) A bar graph from flow cytometric analyses using PBMCs from four individual donors. The mean using ± SEM are shown. * p < 0.01.

Article Snippet: Phenotypes of the clones were evaluated by western blotting using anti-HE4 antibody (Origene, TA801137; ).

Techniques: Expressing, Incubation

Journal: Frontiers in Pharmacology

Article Title: Human Epididymis Secretory Protein 4 (HE4) Compromises Cytotoxic Mononuclear Cells via Inducing Dual Specificity Phosphatase 6

doi: 10.3389/fphar.2019.00216

Figure Lengend Snippet: HE4 suppresses Erk1/2 phosphorylation in CD8 + and CD56 + cells via DUSP6 induction. Two-color flow cytometric analysis of PBMC following 24-hr incubation with PBS (CTR), rHE4 (0.01 mg /mL) and BCI (1 mM) as indicated. (A) 2-D scatterplots of phosphor-Erk1/2 (Alexa Fluor 647) and CD8 or CD56 (FITC) are shown. (B) Mean ± SEM from analyses of phosphor-Erk1/2 positive cells from four individual donors are shown in the bar graph. (C) Immunoblotting for phosphor-Erk1/2 in CD56 + NK92MI, CD8 + TALL-104 and CD4 + H9 cells following 1-h incubation with the conditioned media from 24-h PBMC culture with rHE4 (0.01 mg/mL) and BCI (1 mM) in the indicated combinations. Blots of total Erk1/2 are shown as loading controls. (D) Bar graph represents the relative band densities to controls. Mean ± SEM are shown ( n = 4). (E) DUSP6 concentrations in cell lysates and culture supernatants (SN) after 24 h incubation with or without rHE4 (0.01 mg/mL). Mean ± SEM are shown ( n = 4). * p < 0.05, ** p < 0.01, n.s., not significant.

Article Snippet: Phenotypes of the clones were evaluated by western blotting using anti-HE4 antibody (Origene, TA801137; ).

Techniques: Phospho-proteomics, Incubation, Western Blot